The cookie settings on this website are set to 'allow all cookies' to give you the very best experience. Please click Accept Cookies to continue to use the site.

DDIT3 Polyclonal Antibody (CAB0854)

(No reviews yet) Write a Review
SKU:
CAB0854
$331.89

Description

Overview

Title:DDIT3 Polyclonal Antibody (CAB0854)
Size:100µL
Code:CAB0854
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.

Immunogen Information

Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human DDIT3 (NP_004074.2).
GeneID:1649
SwissProt:P35638
Synonyms:DDIT3; CEBPZ; CHOP; CHOP-10; CHOP10; GADD153; C/EBPzeta; DNA damage inducible transcript 3
Calculated MW:19kDa/21kDa
Observed MW:27kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:Mouse testis, Mouse brain
Cellular Location:Cytoplasm, Nucleus

Antibody Images

Western blot - DDIT3 Polyclonal Antibody (CAB0854)

Western blot analysis of extracts of various cell lines, using DDIT3 antibody (CAB0854) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 20s.

Datasheets

Antibody DatasheetAntibody MSDS

View AllClose

Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
View AllClose