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DAD1 Polyclonal Antibody (CAB14723)

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SKU:
CAB14723
$331.89

Description

Overview

Title:DAD1 Polyclonal Antibody (CAB14723)
Size:100µL
Code:CAB14723
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes.

Immunogen Information

Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DAD1 (NP_001335.1).
GeneID:1603
SwissProt:P61803
Synonyms:DAD1; OST2; defender against cell death 1
Calculated MW:12kDa
Observed MW:22kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:A-431, Mouse thymus, Mouse brain, Mouse kidney, Mouse liver, Rat testis
Cellular Location:Endoplasmic reticulum membrane, Multi-pass membrane protein

Antibody Images

Western blot - DAD1 Polyclonal Antibody (CAB14723)

Western blot analysis of extracts of various cell lines, using DAD1 antibody (CAB14723) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5min.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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