Cathepsin D Polyclonal Antibody (CAB0041)

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CAB0041
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Description

Antibody DatasheetAntibody MSDS

Cathepsin D Polyclonal Antibody (CAB0041)

Overview

Title:CTSD Polyclonal Antibody (CAB0041)
Size:100µL
Code:CAB0041
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the A1 family of peptidases. The encoded preproprotein is proteolytically processed to generate multiple protein products. These products include the cathepsin D light and heavy chains, which heterodimerize to form the mature enzyme. This enzyme exhibits pepsin-like activity and plays a role in protein turnover and in the proteolytic activation of hormones and growth factors. Mutations in this gene play a causal role in neuronal ceroid lipofuscinosis-10 and may be involved in the pathogenesis of several other diseases, including breast cancer and possibly Alzheimer's disease.

Immunogen Information

Immunogen:A synthetic peptide of human CTSD
GeneID:1509
SwissProt:P07339
Synonyms:CTSD; CLN10; CPSD; HEL-S-130P; cathepsin D
Calculated MW:44kDa
Observed MW:50kDa

Applications

Reactivity:Human
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:20 - 1:50
Storage Buffer:Store at 4°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:HepG2
Cellular Location:Lysosome, Melanosome, Secreted, extracellular space

Antibody Images

Western blot - CTSD Polyclonal Antibody (CAB0041)

Western blot analysis of extracts of HepG2 cells, using CTSD antibody (CAB0041).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Cathepsin D Polyclonal Antibody (CAB0041) Protein Information

UniProt Protein Function:CTSD: Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. Consists of a light chain and a heavy chain. Belongs to the peptidase A1 family.
UniProt Protein Details:

Protein type:Autophagy; Motility/polarity/chemotaxis; Protease; EC 3.4.23.5

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular matrix; extracellular space; lysosomal lumen; lysosome; melanosome; extracellular region

Molecular Function:protein binding; aspartic-type endopeptidase activity

Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class II; proteolysis

Disease: Ceroid Lipofuscinosis, Neuronal, 10

NCBI Summary:This gene encodes a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. This proteinase, which is a member of the peptidase C1 family, has a specificity similar to but narrower than that of pepsin A. Transcription of this gene is initiated from several sites, including one which is a start site for an estrogen-regulated transcript. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease. [provided by RefSeq, Jul 2008]
UniProt Code:P07339
NCBI GenInfo Identifier:115717
NCBI Gene ID:1509
NCBI Accession:P07339.1
UniProt Secondary Accession:P07339,Q6IB57,
UniProt Related Accession:P07339
Molecular Weight:44,552 Da
NCBI Full Name:Cathepsin D
NCBI Synonym Full Names:cathepsin D
NCBI Official Symbol:CTSD  
NCBI Official Synonym Symbols:CPSD; CLN10; HEL-S-130P  
NCBI Protein Information:cathepsin D; lysosomal aspartyl protease; lysosomal aspartyl peptidase; ceroid-lipofuscinosis, neuronal 10; epididymis secretory sperm binding protein Li 130P
UniProt Protein Name:Cathepsin D
Protein Family:Cathepsin
UniProt Gene Name:CTSD  
UniProt Entry Name:CATD_HUMAN
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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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