CNOT7 Polyclonal Antibody (CAB8959)

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CAB8959
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Description

Antibody DatasheetAntibody MSDS

CNOT7 Polyclonal Antibody (CAB8959)

Overview

Title:CNOT7 Polyclonal Antibody (CAB8959)
Size:100µL
Code:CAB8959
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene binds to an anti-proliferative protein, B-cell translocation protein 1, which negatively regulates cell proliferation. Binding of the two proteins, which is driven by phosphorylation of the anti-proliferative protein, causes signaling events in cell division that lead to changes in cell proliferation associated with cell-cell contact. The encoded protein downregulates the innate immune response and therefore provides a therapeutic target for enhancing its antimicrobial activity against foreign agents. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and X.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 90-160 of human CNOT7 (NP_037486.2).
GeneID:29883
SwissProt:Q9UIV1
Synonyms:CNOT7; CAF-1; CAF1; Caf1a; hCAF-1; CCR4-NOT transcription complex subunit 7
Calculated MW:28kDa/32kDa
Observed MW:36kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB
Recommended Dilution:WB 1:200 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:22Rv1, U-87MG, HT-29, Raji, HeLa, Mouse lung, Mouse brain, Rat lung
Cellular Location:Cytoplasm, Nucleus, P-body

Antibody Images

Western blot - CNOT7 Polyclonal Antibody (CAB8959)

Western blot analysis of extracts of various cell lines, using CNOT7 antibody (CAB8959) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

CNOT7 Polyclonal Antibody (CAB8959) Protein Information

UniProt Protein Function:CNOT7: Ubiquitous transcription factor required for a diverse set of processes. It is a component of the CCR4 complex involved in the control of gene expression. Belongs to the CAF1 family.
UniProt Protein Details:

Protein type:EC 3.1.13.4; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 8p22-p21.3

Cellular Component: membrane; nucleus; cytosol; CCR4-NOT complex

Molecular Function:protein binding; signal transducer activity; 3'-5'-exoribonuclease activity; poly(A)-specific ribonuclease activity; exoribonuclease activity; RNA binding; metal ion binding; transcription factor activity

Biological Process: deadenylation-dependent decapping; transcription, DNA-dependent; signal transduction; miRNA-mediated gene silencing; regulation of translation; negative regulation of cell proliferation; poly(A) tail shortening; carbohydrate metabolic process; positive regulation of cell proliferation; gene expression; RNA-mediated gene silencing; positive regulation of transcription from RNA polymerase II promoter; cytoplasmic mRNA processing body assembly; mRNA catabolic process, deadenylation-dependent decay

NCBI Summary:The protein encoded by this gene binds to an anti-proliferative protein, B-cell translocation protein 1, which negatively regulates cell proliferation. Binding of the two proteins, which is driven by phosphorylation of the anti-proliferative protein, causes signaling events in cell division that lead to changes in cell proliferation associated with cell-cell contact. The encoded protein downregulates the innate immune response and therefore provides a therapeutic target for enhancing its antimicrobial activity against foreign agents. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Sep 2014]
UniProt Code:Q9UIV1
NCBI GenInfo Identifier:41713629
NCBI Gene ID:29883
NCBI Accession:Q9UIV1.3
UniProt Secondary Accession:Q9UIV1,Q7Z530, A8MZM5, B3KMP1, B3KN35, D3DSP6, G3V108
UniProt Related Accession:Q9UIV1
Molecular Weight:28,228 Da
NCBI Full Name:CCR4-NOT transcription complex subunit 7
NCBI Synonym Full Names:CCR4-NOT transcription complex, subunit 7
NCBI Official Symbol:CNOT7  
NCBI Official Synonym Symbols:CAF1; Caf1a; hCAF-1  
NCBI Protein Information:CCR4-NOT transcription complex subunit 7; CAF-1; BTG1 binding factor 1; BTG1-binding factor 1; CCR4-associated factor 1; carbon catabolite repressor protein (CCR4)-associative factor 1
UniProt Protein Name:CCR4-NOT transcription complex subunit 7
UniProt Synonym Protein Names:BTG1-binding factor 1; CCR4-associated factor 1; CAF-1; Caf1a
Protein Family:CCR4-NOT transcription complex
UniProt Gene Name:CNOT7  
UniProt Entry Name:CNOT7_HUMAN
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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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