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CLTC Polyclonal Antibody (CAB12423)

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CAB12423
  • Western blot - CLTC Polyclonal Antibody (CAB12423)
  • Immunofluorescence - CLTC Polyclonal Antibody (CAB12423)
$331.89

Description

Overview

Title:CLTC Polyclonal Antibody (CAB12423)
Size:100µL
Code:CAB12423
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1451-1675 of human CLTC (NP_004850.1).
GeneID:1213
SwissProt:Q00610
Synonyms:CLTC; CHC; CHC17; CLH-17; CLTCL2; Hc; clathrin heavy chain
Calculated MW:187kDa/191kDa
Observed MW:191kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IF
Recommended Dilution:WB 1:500 - 1:2000; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:HeLa, HepG2, NIH/3T3, Mouse brain, Mouse lung, Rat brain
Cellular Location:Cytoplasmic side, Cytoplasmic vesicle membrane, Melanosome, Membrane, Peripheral membrane protein, coated pit

Antibody Images

Western blot - CLTC Polyclonal Antibody (CAB12423)

Western blot analysis of extracts of various cell lines, using CLTC antibody (CAB12423) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Immunofluorescence - CLTC Polyclonal Antibody (CAB12423)

Immunofluorescence analysis of HeLa cells using CLTC antibody (CAB12423) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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