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CISD2 Polyclonal Antibody (CAB5231)

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SKU:
CAB5231
€299.00

Description

Overview

Title:CISD2 Polyclonal Antibody (CAB5231)
Size:100µL
Code:CAB5231
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is a zinc finger protein that localizes to the endoplasmic reticulum. The encoded protein binds an iron/sulfur cluster and may be involved in calcium homeostasis. Defects in this gene are a cause of Wolfram syndrome 2.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 61-135 of human CISD2 (NP_001008389.1).
GeneID:493856
SwissProt:Q8N5K1
Synonyms:CISD2; ERIS; Miner1; NAF-1; WFS2; ZCD2; CDGSH iron sulfur domain 2
Calculated MW:15kDa
Observed MW:15kDa

Applications

Reactivity:Human, Rat
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:HT-29, 293T, A-549, SH-SY5Y, K-562, Rat lung
Cellular Location:Endoplasmic reticulum membrane, Mitochondrion outer membrane, Single-pass membrane protein

Antibody Images

Western blot - CISD2 Polyclonal Antibody (CAB5231)

Western blot analysis of extracts of various cell lines, using CISD2 antibody (CAB5231) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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