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CHGA Polyclonal Antibody (CAB1668)

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CAB1668
  • Western blot - CHGA Polyclonal Antibody (CAB1668)
  • Immunohistochemistry - CHGA Polyclonal Antibody (CAB1668)
  • Immunohistochemistry - CHGA Polyclonal Antibody (CAB1668)
$331.89

Description

Overview

Title:CHGA Polyclonal Antibody (CAB1668)
Size:100µL
Code:CAB1668
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 230-380 of human CHGA (NP_001266.1).
GeneID:1113
SwissProt:P10645
Synonyms:CHGA; CGA; chromogranin-A
Calculated MW:50kDa
Observed MW:50kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:LO2, SGC7901, Mouse kidney, Rat kidney
Cellular Location:Cytoplasmic vesicle, Secreted, Secreted, secretory vesicle, secretory vesicle lumen, secretory vesicle membrane

Antibody Images

Western blot - CHGA Polyclonal Antibody (CAB1668)

Western blot analysis of extracts of various cell lines, using CHGA antibody (CAB1668) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Immunohistochemistry - CHGA Polyclonal Antibody (CAB1668)

Immunohistochemistry of paraffin-embedded mouse brain using CHGA antibody (CAB1668) at dilution of 1:150 (40x lens).

Immunohistochemistry - CHGA Polyclonal Antibody (CAB1668)

Immunohistochemistry of paraffin-embedded mouse spleen using CHGA antibody (CAB1668) at dilution of 1:150 (40x lens).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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