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CELF2 Polyclonal Antibody (CAB6016)

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SKU:
CAB6016
$331.89

Description

Overview

Title:CELF2 Polyclonal Antibody (CAB6016)
Size:100µL
Code:CAB6016
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 422-521 of human CELF2 (NP_006552.3).
GeneID:10659
SwissProt:O95319
Synonyms:CELF2; BRUNOL3; CELF-2; CUG-BP2; CUGBP2; ETR-3; ETR3; NAPOR; CUGBP Elav-like family member 2
Calculated MW:52kDa/54kDa/55kDa
Observed MW:60kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB
Recommended Dilution:WB 1:200 - 1:500
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:MCF-7, HeLa
Cellular Location:Cytoplasm, Nucleus

Antibody Images

Western blot - CELF2 Polyclonal Antibody (CAB6016)

Western blot analysis of extracts of various cell lines, using CELF2 antibody (CAB6016) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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