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CELF1 Polyclonal Antibody (CAB5958)

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SKU:
CAB5958
$331.89

Description

Overview

Title:CELF1 Polyclonal Antibody (CAB5958)
Size:100µL
Code:CAB5958
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. This gene may play a role in myotonic dystrophy type 1 (DM1) via interactions with the dystrophia myotonica-protein kinase (DMPK) gene. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 108-483 of human CELF1 (NP_941989.1).
GeneID:10658
SwissProt:Q92879
Synonyms:CELF1; BRUNOL2; CUG-BP; CUGBP; CUGBP1; EDEN-BP; NAB50; NAPOR; hNab50; CUGBP Elav-like family member 1
Calculated MW:50kDa/51kDa/52kDa/55kDa
Observed MW:52kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB
Recommended Dilution:WB 1:200 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:U-87MG, SH-SY5Y, HepG2, NIH/3T3, HeLa, Mouse liver, Mouse testis
Cellular Location:Cytoplasm, Nucleus

Antibody Images

Western blot - CELF1 Polyclonal Antibody (CAB5958)

Western blot analysis of extracts of various cell lines, using CELF1 antibody (CAB5958) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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