CDK9 Polyclonal Antibody (CAB1564)
- SKU:
- CAB1564
Description
| Title: | CDK9 Polyclonal Antibody (CAB1564) | |
| Size: | 100µL | |
| Code: | CAB1564 | |
| Host Species: | Rabbit | |
| Purification: | Affinity purification | |
| Isotype: | IgG |
Background
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.
Immunogen Information
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 153-372 of human CDK9 (NP_001252.1). |
| GeneID: | 1025 |
| SwissProt: | P50750 |
| Synonyms: | CDK9; C-2k; CDC2L4; CTK1; PITALRE; TAK; cyclin-dependent kinase 9 |
| Calculated MW: | 42kDa/53kDa |
| Observed MW: | 40kDa |
Applications
| Reactivity: | Human, Mouse |
| Tested Applications: | WB; IHC; IF; IP |
| Recommended Dilution: | WB 1:1000 - 1:3000; IHC 1:50 - 1:200; IF 1:50 - 1:200; IP 1:50 - 1:100 |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Application Key: | Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation |
| Positive Samples: | A549, HeLa, U-251MG, Raji, BT-474, Mouse spleen |
| Cellular Location: | Cytoplasm, Nucleus, PML body |
Antibody Images
| Western blot analysis of extracts of various cell lines, using CDK9 antibody (CAB1564) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. |
| Immunofluorescence analysis of U2OS cells using CDK9 antibody (CAB1564). Blue: DAPI for nuclear staining. |
| Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug CDK9 antibody (CAB1564). Western blot was performed from the immunoprecipitate using CDK9 antibody (CAB1564) at a dilition of 1:1000. |
Datasheets
Additional Information
Antibody Type: |
Polyclonal |
Application: |
WB |
Application: |
IHC |
Application: |
IF |
Application: |
IP |
Reactivity: |
Human |
Reactivity: |
Mouse |
Host Species: |
Rabbit |
Isotype: |
IgG |
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