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CDK9 Polyclonal Antibody (CAB1564)

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CAB1564
  • Western blot - CDK9 Polyclonal Antibody (CAB1564)
  • Immunofluorescence - CDK9 Polyclonal Antibody (CAB1564)
  • Immunoprecipitation - CDK9 Polyclonal Antibody (CAB1564)
$331.89

Description

Overview

Title:CDK9 Polyclonal Antibody (CAB1564)
Size:100µL
Code:CAB1564
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 153-372 of human CDK9 (NP_001252.1).
GeneID:1025
SwissProt:P50750
Synonyms:CDK9; C-2k; CDC2L4; CTK1; PITALRE; TAK; cyclin-dependent kinase 9
Calculated MW:42kDa/53kDa
Observed MW:40kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IHC; IF; IP
Recommended Dilution:WB 1:1000 - 1:3000; IHC 1:50 - 1:200; IF 1:50 - 1:200; IP 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive Samples:A549, HeLa, U-251MG, Raji, BT-474, Mouse spleen
Cellular Location:Cytoplasm, Nucleus, PML body

Antibody Images

Western blot - CDK9 Polyclonal Antibody (CAB1564)

Western blot analysis of extracts of various cell lines, using CDK9 antibody (CAB1564) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence - CDK9 Polyclonal Antibody (CAB1564)

Immunofluorescence analysis of U2OS cells using CDK9 antibody (CAB1564). Blue: DAPI for nuclear staining.

Immunoprecipitation - CDK9 Polyclonal Antibody (CAB1564)

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug CDK9 antibody (CAB1564). Western blot was performed from the immunoprecipitate using CDK9 antibody (CAB1564) at a dilition of 1:1000.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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