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CDK1 Polyclonal Antibody (CAB2861)

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CAB2861
  • Western blot - CDK1 Polyclonal Antibody (CAB2861)
  • Immunohistochemistry - CDK1 Polyclonal Antibody (CAB2861)
  • Immunohistochemistry - CDK1 Polyclonal Antibody (CAB2861)
  • Immunofluorescence - CDK1 Polyclonal Antibody (CAB2861)
$331.89

Description

Overview

Title:CDK1 Polyclonal Antibody (CAB2861)
Size:100µL
Code:CAB2861
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

Immunogen:A synthetic peptide of human CDK1
GeneID:983
SwissProt:P06493
Synonyms:CDK1; CDC2; CDC28A; P34CDC2; cyclin-dependent kinase 1
Calculated MW:27kDa/34kDa
Observed MW:33kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IHC; IF; IP
Recommended Dilution:WB 1:200 - 1:1000; IHC 1:50 - 1:100; IF 1:50 - 1:100; IP 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive Samples:HeLa, Jurkat, Mouse thymus
Cellular Location:Cytoplasm, Mitochondrion, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle

Antibody Images

Western blot - CDK1 Polyclonal Antibody (CAB2861)

Western blot analysis of extracts of various cell lines, using CDK1 antibody (CAB2861) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Immunohistochemistry - CDK1 Polyclonal Antibody (CAB2861)

Immunohistochemistry of paraffin-embedded human prostate using CDK1 antibody (CAB2861) at dilution of 1:100 (40x lens).

Immunohistochemistry - CDK1 Polyclonal Antibody (CAB2861)

Immunohistochemistry of paraffin-embedded human lung cancer using CDK1 antibody (CAB2861) at dilution of 1:100 (40x lens).

Immunofluorescence - CDK1 Polyclonal Antibody (CAB2861)

Immunofluorescence analysis of A-549 cells using CDK1 antibody (CAB2861). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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