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CDC45 Polyclonal Antibody (CAB2047)

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SKU:
CAB2047
  • Western blot - CDC45 Polyclonal Antibody (CAB2047)
  • Immunohistochemistry - CDC45 Polyclonal Antibody (CAB2047)
  • Immunohistochemistry - CDC45 Polyclonal Antibody (CAB2047)
€299.00

Description

Overview

Title:CDC45 Polyclonal Antibody (CAB2047)
Size:100µL
Code:CAB2047
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human CDC45 (NP_003495.1).
GeneID:8318
SwissProt:O75419
Synonyms:CDC45; CDC45L; CDC45L2; MGORS7; PORC-PI-1; cell division cycle 45
Calculated MW:60kDa/65kDa/68kDa
Observed MW:60kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:U-251MG, HeLa, A-549, HL-60, HT-29, Mouse thymus
Cellular Location:Cytoplasm, Nucleus

Antibody Images

Western blot - CDC45 Polyclonal Antibody (CAB2047)

Western blot analysis of extracts of various cell lines, using cdc45 antibody (CAB2047) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.

Immunohistochemistry - CDC45 Polyclonal Antibody (CAB2047)

Immunohistochemistry of paraffin-embedded mouse stomach using cdc45 antibody (CAB2047) at dilution of 1:100 (40x lens).

Immunohistochemistry - CDC45 Polyclonal Antibody (CAB2047)

Immunohistochemistry of paraffin-embedded human stomach using cdc45 antibody (CAB2047) at dilution of 1:100 (40x lens).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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