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CDC25C Polyclonal Antibody (CAB1672)

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SKU:
CAB1672
€299.00

Description

Overview

Title:CDC25C Polyclonal Antibody (CAB1672)
Size:100µL
Code:CAB1672
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 174-473 of human CDC25C (NP_001781.2).
GeneID:995
SwissProt:P30307
Synonyms:CDC25C; CDC25; PPP1R60; cell division cycle 25C
Calculated MW:45kDa/48kDa/50kDa/53kDa
Observed MW:70kDa

Applications

Reactivity:Human
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:K-562, HepG2, Raji
Cellular Location:Nucleus

Antibody Images

Western blot - CDC25C Polyclonal Antibody (CAB1672)

Western blot analysis of extracts of various cell lines, using CDC25C antibody (CAB1672) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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