The cookie settings on this website are set to 'allow all cookies' to give you the very best experience. Please click Accept Cookies to continue to use the site.

CDC23 Polyclonal Antibody (CAB15116)

(No reviews yet) Write a Review
SKU:
CAB15116
€299.00

Description

Overview

Title:CDC23 Polyclonal Antibody (CAB15116)
Size:100µL
Code:CAB15116
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction.

Immunogen Information

Immunogen:A synthetic peptide corresponding to a sequence within amino acids 50-150 of human CDC23 (NP_004652.2).
GeneID:8697
SwissProt:Q9UJX2
Synonyms:CDC23; ANAPC8; APC8; CUT23; cell division cycle 23
Calculated MW:16kDa/55kDa/68kDa
Observed MW:69kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:U-87MG, 293T, HeLa, Jurkat, HepG2, NIH/3T3, mouse liver, mouse kidney, rat liver
Cellular Location:N/A

Antibody Images

Western blot - CDC23 Polyclonal Antibody (CAB15116)

Western blot analysis of extracts of various cell lines, using CDC23 antibody (CAB15116) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

Datasheets

Antibody DatasheetAntibody MSDS

View AllClose

Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
View AllClose