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CDC16 Polyclonal Antibody (CAB7197)

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CAB7197
  • Western blot - CDC16 Polyclonal Antibody (CAB7197)
  • Immunohistochemistry - CDC16 Polyclonal Antibody (CAB7197)
  • Immunohistochemistry - CDC16 Polyclonal Antibody (CAB7197)
  • Immunofluorescence - CDC16 Polyclonal Antibody (CAB7197)
€299.00

Description

Overview

Title:CDC16 Polyclonal Antibody (CAB7197)
Size:100µL
Code:CAB7197
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene functions as a protein ubiquitin ligase and is a component of the multiprotein APC complex. The APC complex is a cyclin degradation system that governs exit from mitosis by targeting cell cycle proteins for degredation by the 26S proteasome. Each component protein of the APC complex is highly conserved among eukaryotic organisms. This protein, and other APC complex proteins, contain a tetratricopeptide repeat (TPR) domain; a protein domain that is often involved in protein-protein interactions and the assembly of multiprotein complexes. Multiple alternatively spliced transcript variants, encoding distinct proteins, have been identified.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 371-620 of human CDC16 (NP_003894.3).
GeneID:8881
SwissProt:Q13042
Synonyms:CDC16; ANAPC6; APC6; CDC16Hs; CUT9; cell division cycle 16
Calculated MW:60kDa/65kDa/71kDa
Observed MW:72kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:Jurkat, HepG2, HeLa, A-549, Mouse brain, Mouse ovary, Rat brain
Cellular Location:Cytoplasm, centrosome, cytoskeleton, microtubule organizing center, spindle

Antibody Images

Western blot - CDC16 Polyclonal Antibody (CAB7197)

Western blot analysis of extracts of various cell lines, using CDC16 antibody (CAB7197) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

Immunohistochemistry - CDC16 Polyclonal Antibody (CAB7197)

Immunohistochemistry of paraffin-embedded human liver cancer using CDC16 antibody (CAB7197) at dilution of 1:100 (40x lens).

Immunohistochemistry - CDC16 Polyclonal Antibody (CAB7197)

Immunohistochemistry of paraffin-embedded human gastric cancer using CDC16 antibody (CAB7197) at dilution of 1:100 (40x lens).

Immunofluorescence - CDC16 Polyclonal Antibody (CAB7197)

Immunofluorescence analysis of A549 cells using CDC16 antibody (CAB7197). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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