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Cyclin-H Polyclonal Antibody (CAB0995)

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CAB0995
  • Western blot - CCNH Polyclonal Antibody (CAB0995)
  • Immunoprecipitation - CCNH Polyclonal Antibody (CAB0995)
€299.00

Description

Overview

Title:CCNH Polyclonal Antibody (CAB0995)
Size:100µL
Code:CAB0995
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. A pseudogene of this gene is found on chromosome 4. Alternate splicing results in multiple transcript variants.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human CCNH (NP_001230.1).
GeneID:902
SwissProt:P51946
Synonyms:CCNH; CAK; CycH; p34; p37; cyclin-H
Calculated MW:37kDa
Observed MW:36kDa

Applications

Reactivity:Human
Tested Applications:WB; IP
Recommended Dilution:WB 1:500 - 1:2000; IP 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunoprecipitation    
Positive Samples:Jurkat, K-562, SW620, HT-29, MCF7
Cellular Location:Nucleus

Antibody Images

Western blot - CCNH Polyclonal Antibody (CAB0995)

Western blot analysis of extracts of various cell lines, using CCNH Antibody (CAB0995).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).

Immunoprecipitation - CCNH Polyclonal Antibody (CAB0995)

Immunoprecipitation analysis of 200ug extracts of K562 cells using 1ug CCNH antibody (CAB0995). Western blot was performed from the immunoprecipitate using CCNH antibody (CAB0995) at a dilition of 1:1000.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IP
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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