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CCNE2 Polyclonal Antibody (CAB7032)

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CAB7032
  • Western blot - CCNE2 Polyclonal Antibody (CAB7032)
  • Immunofluorescence - CCNE2 Polyclonal Antibody (CAB7032)
€299.00

Description

Overview

Title:CCNE2 Polyclonal Antibody (CAB7032)
Size:100µL
Code:CAB7032
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2. This cyclin has been shown to specifically interact with CIP/KIP family of CDK inhibitors, and plays a role in cell cycle G1/S transition. The expression of this gene peaks at the G1-S phase and exhibits a pattern of tissue specificity distinct from that of cyclin E1. A significantly increased expression level of this gene was observed in tumor-derived cells.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 255-404 of human CCNE2 (NP_477097.1).
GeneID:9134
SwissProt:O96020
Synonyms:CCNE2; CYCE2; cyclin E2
Calculated MW:41kDa/46kDa
Observed MW:47kDa

Applications

Reactivity:Human
Tested Applications:WB; IF
Recommended Dilution:WB 1:500 - 1:2000; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:Jurkat
Cellular Location:Nucleus

Antibody Images

Western blot - CCNE2 Polyclonal Antibody (CAB7032)

Western blot analysis of extracts of Jurkat cells, using CCNE2 antibody (CAB7032).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence - CCNE2 Polyclonal Antibody (CAB7032)

Immunofluorescence analysis of MCF7 cells using CCNE2 antibody (CAB7032).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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