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CCNE2 Polyclonal Antibody (CAB4272)

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SKU:
CAB4272
€299.00

Description

Overview

Title:CCNE2 Polyclonal Antibody (CAB4272)
Size:100µL
Code:CAB4272
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2. This cyclin has been shown to specifically interact with CIP/KIP family of CDK inhibitors, and plays a role in cell cycle G1/S transition. The expression of this gene peaks at the G1-S phase and exhibits a pattern of tissue specificity distinct from that of cyclin E1. A significantly increased expression level of this gene was observed in tumor-derived cells.

Immunogen Information

Immunogen:A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human CCNE2 (NP_477097.1).
GeneID:9134
SwissProt:O96020
Synonyms:CCNE2; CYCE2; cyclin E2
Calculated MW:41kDa/46kDa
Observed MW:40kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:Mouse brain, Rat brain
Cellular Location:Nucleus

Antibody Images

Western blot - CCNE2 Polyclonal Antibody (CAB4272)

Western blot analysis of extracts of various cell lines, using CCNE2 antibody (CAB4272) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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