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CCND2 Polyclonal Antibody (CAB13284)

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CAB13284
  • Western blot - CCND2 Polyclonal Antibody (CAB13284)
  • Immunofluorescence - CCND2 Polyclonal Antibody (CAB13284)
€299.00

Description

Overview

Title:CCND2 Polyclonal Antibody (CAB13284)
Size:100µL
Code:CAB13284
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK4 or CDK6 and functions as a regulatory subunit of the complex, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. Knockout studies of the homologous gene in mouse suggest the essential roles of this gene in ovarian granulosa and germ cell proliferation. High level expression of this gene was observed in ovarian and testicular tumors. Mutations in this gene are associated with megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 3 (MPPH3).

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-289 of human CCND2 (NP_001750.1).
GeneID:894
SwissProt:P30279
Synonyms:CCND2; KIAK0002; MPPH3; cyclin D2
Calculated MW:24kDa/33kDa
Observed MW:36kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:HeLa, 293T, NIH/3T3
Cellular Location:Cytoplasm, Membrane, Nucleus

Antibody Images

Western blot - CCND2 Polyclonal Antibody (CAB13284)

Western blot analysis of extracts of various cell lines, using CCND2 antibody (CAB13284) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Immunofluorescence - CCND2 Polyclonal Antibody (CAB13284)

Immunofluorescence analysis of U2OS cells using CCND2 antibody (CAB13284). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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