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CCND1 Polyclonal Antibody (CAB11310)

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SKU:
CAB11310
€299.00

Description

Overview

Title:CCND1 Polyclonal Antibody (CAB11310)
Size:100µL
Code:CAB11310
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.

Immunogen Information

Immunogen:A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human CCND1 (NP_444284.1).
GeneID:595
SwissProt:P24385
Synonyms:CCND1; BCL1; D11S287E; PRAD1; U21B31; cyclin D1
Calculated MW:33kDa
Observed MW:37kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IF; IP
Recommended Dilution:WB 1:500 - 1:2000; IF 1:50 - 1:200; IP 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    Immunoprecipitation    
Positive Samples:U-87MG, HepG2, SW480, HT-1080
Cellular Location:Cytoplasm, Membrane, Nucleus

Antibody Images

Western blot - CCND1 Polyclonal Antibody (CAB11310)

Western blot analysis of extracts of various cell lines, using CCND1 antibody (CAB11310) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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