The cookie settings on this website are set to 'allow all cookies' to give you the very best experience. Please click Accept Cookies to continue to use the site.

CCNB1 Polyclonal Antibody (CAB16038)

(No reviews yet) Write a Review
SKU:
CAB16038
  • Western blot - CCNB1 Polyclonal Antibody (CAB16038)
  • Immunohistochemistry - CCNB1 Polyclonal Antibody (CAB16038)
  • Immunohistochemistry - CCNB1 Polyclonal Antibody (CAB16038)
  • Immunohistochemistry - CCNB1 Polyclonal Antibody (CAB16038)
  • Immunohistochemistry - CCNB1 Polyclonal Antibody (CAB16038)
  • Immunofluorescence - CCNB1 Polyclonal Antibody (CAB16038)
  • Immunoprecipitation - CCNB1 Polyclonal Antibody (CAB16038)
€299.00

Description

Overview

Title:CCNB1 Polyclonal Antibody (CAB16038)
Size:100µL
Code:CAB16038
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Immunogen Information

Immunogen:A synthetic peptide of human CCNB1
GeneID:891
SwissProt:P14635
Synonyms:CCNB1; CCNB; cyclin B1
Calculated MW:44kDa/48kDa
Observed MW:55kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF; IP
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200; IP 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive Samples:NIH/3T3, Rat spleen, Rat thymus
Cellular Location:Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center

Antibody Images

Western blot - CCNB1 Polyclonal Antibody (CAB16038)

Western blot analysis of extracts of various cell lines, using CCNB1 antibody (CAB16038) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Immunohistochemistry - CCNB1 Polyclonal Antibody (CAB16038)

Immunohistochemistry of paraffin-embedded rat testis using CCNB1 antibody (CAB16038) at dilution of 1:100 (40x lens).

Immunohistochemistry - CCNB1 Polyclonal Antibody (CAB16038)

Immunohistochemistry of paraffin-embedded human lung cancer using CCNB1 antibody (CAB16038) at dilution of 1:100 (40x lens).

Immunohistochemistry - CCNB1 Polyclonal Antibody (CAB16038)

Immunohistochemistry of paraffin-embedded human breast cancer using CCNB1 antibody (CAB16038) at dilution of 1:100 (40x lens).

Immunohistochemistry - CCNB1 Polyclonal Antibody (CAB16038)

Immunohistochemistry of paraffin-embedded mouse testis using CCNB1 antibody (CAB16038) at dilution of 1:100 (40x lens).

Immunofluorescence - CCNB1 Polyclonal Antibody (CAB16038)

Immunofluorescence analysis of 293T cells using CCNB1 antibody (CAB16038) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation - CCNB1 Polyclonal Antibody (CAB16038)

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug CCNB1 antibody (CAB16038). Western blot was performed from the immunoprecipitate using CCNB1 antibody (CAB16038) at a dilition of 1:1000.

Datasheets

Antibody DatasheetAntibody MSDS

View AllClose

Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
View AllClose