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Cyclin-A2 Polyclonal Antibody (CAB7632)

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SKU:
CAB7632
  • Western blot - CCNA2 Polyclonal Antibody (CAB7632)
  • Immunohistochemistry - CCNA2 Polyclonal Antibody (CAB7632)
  • Immunohistochemistry - CCNA2 Polyclonal Antibody (CAB7632)
  • Immunofluorescence - CCNA2 Polyclonal Antibody (CAB7632)
€299.00

Description

Overview

Title:CCNA2 Polyclonal Antibody (CAB7632)
Size:100µL
Code:CAB7632
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.

Immunogen Information

Immunogen:A synthetic peptide of human CCNA2
GeneID:890
SwissProt:P20248
Synonyms:CCNA2; CCN1; CCNA; cyclin-A2
Calculated MW:48kDa
Observed MW:49kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:Mouse brain, Mouse liver, Mouse spleen, Mouse eye, Rat spinal cord
Cellular Location:Cytoplasm, Nucleus

Antibody Images

Western blot - CCNA2 Polyclonal Antibody (CAB7632)

Western blot analysis of extracts of various cell lines, using CCNA2 antibody (CAB7632) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 90s.

Immunohistochemistry - CCNA2 Polyclonal Antibody (CAB7632)

Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using CCNA2 antibody (CAB7632) at dilution of 1:100 (40x lens).

Immunohistochemistry - CCNA2 Polyclonal Antibody (CAB7632)

Immunohistochemistry of paraffin-embedded mouse kidney using CCNA2 antibody (CAB7632) at dilution of 1:100 (40x lens).

Immunofluorescence - CCNA2 Polyclonal Antibody (CAB7632)

Immunofluorescence analysis of U2OS cells using CCNA2 antibody (CAB7632) at dilution of 1:100. Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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