The cookie settings on this website are set to 'allow all cookies' to give you the very best experience. Please click Accept Cookies to continue to use the site.

Casp7 Polyclonal Antibody (CAB1524)

(No reviews yet) Write a Review
SKU:
CAB1524
  • Western blot - Casp7 Polyclonal Antibody (CAB1524)
  • Immunohistochemistry - Casp7 Polyclonal Antibody (CAB1524)
  • Immunohistochemistry - Casp7 Polyclonal Antibody (CAB1524)
  • Immunofluorescence - Casp7 Polyclonal Antibody (CAB1524)
$331.89

Description

Overview

Title:Casp7 Polyclonal Antibody (CAB1524)
Size:100µL
Code:CAB1524
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 24-303 of human Casp7 (NP_001218.1).
GeneID:840
SwissProt:P55210
Synonyms:CASP7; CASP-7; CMH-1; ICE-LAP3; LICE2; MCH3; caspase-7
Calculated MW:28kDa/31kDa/34kDa/37kDa
Observed MW:37kDa

Applications

Reactivity:Human, Rat
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:293T, Mouse liver, Mouse lung, Mouse intestine
Cellular Location:Cytoplasm

Antibody Images

Western blot - Casp7 Polyclonal Antibody (CAB1524)

Western blot analysis of extracts of various cell lines, using CASP7 antibody (CAB1524) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry - Casp7 Polyclonal Antibody (CAB1524)

Immunohistochemistry of paraffin-embedded rat heart using CASP7 antibody (CAB1524) at dilution of 1:100 (40x lens).

Immunohistochemistry - Casp7 Polyclonal Antibody (CAB1524)

Immunohistochemistry of paraffin-embedded human lung cancer using CASP7 antibody (CAB1524) at dilution of 1:100 (40x lens).

Immunofluorescence - Casp7 Polyclonal Antibody (CAB1524)

Immunofluorescence analysis of HeLa cells using Casp7 antibody (CAB1524).

Datasheets

Antibody DatasheetAntibody MSDS

View AllClose

Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
View AllClose