CAPN2 Monoclonal Antibody (CAB0900)

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CAB0900
€299.00
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Description

Antibody DatasheetAntibody MSDS

CAPN2 Monoclonal Antibody (CAB0900)

Overview

Title:CAPN2 Monoclonal Antibody (CAB0900)
Size:100µL
Code:CAB0900
Host Species:Mouse
Purification:Affinity purification
Isotype:IgG

Background

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

Immunogen:A synthetic peptide of human CAPN2
GeneID:824
SwissProt:P17655
Synonyms:CAPN2; CANP2; CANPL2; CANPml; mCANP; calpain 2
Calculated MW:71kDa/79kDa
Observed MW:80kDa

Applications

Reactivity:Human
Tested Applications:WB; IHC; FC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:100; FC 1:20 - 1:50
Storage Buffer:Store at 4°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Flow Cytometry    
Positive Samples:Y79
Cellular Location:Cell membrane, Cytoplasm

Antibody Images

Western blot - CAPN2 Monoclonal Antibody (CAB0900)

Western blot analysis of extracts of Y79 cells, using CAPN2 antibody (CAB0900).Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

CAPN2 Monoclonal Antibody (CAB0900) Protein Information

UniProt Protein Function:Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (PubMed:17650508). Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs.
NCBI Summary:The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
UniProt Code:P17655
NCBI GenInfo Identifier:317373596
NCBI Gene ID:824
NCBI Accession:P17655.6
UniProt Secondary Accession:P17655,Q16738, Q6PJT3, Q8WU26, Q9HBB1, A6NDG7, B7ZA96 E7ES58,
UniProt Related Accession:P17655
Molecular Weight:71,476 Da
NCBI Full Name:Calpain-2 catalytic subunit
NCBI Synonym Full Names:calpain 2
NCBI Official Symbol:CAPN2  
NCBI Official Synonym Symbols:CANP2; mCANP; CANPL2; CANPml  
NCBI Protein Information:calpain-2 catalytic subunit
UniProt Protein Name:Calpain-2 catalytic subunit
UniProt Synonym Protein Names:Calcium-activated neutral proteinase 2; CANP 2; Calpain M-type; Calpain large polypeptide L2; Calpain-2 large subunit; Millimolar-calpain; M-calpain
Protein Family:Calpain
UniProt Gene Name:CAPN2  
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Additional Information

Antibody Type:
Monoclonal
Application:
WB
Application:
IHC
Application:
FC
Reactivity:
Human
Host Species:
Mouse
Isotype:
IgG
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