CAMK4 Polyclonal Antibody (CAB5304)
- SKU:
- CAB5304
Description
| Title: | CAMK4 Polyclonal Antibody (CAB5304) | |
| Size: | 100µL | |
| Code: | CAB5304 | |
| Host Species: | Rabbit | |
| Purification: | Affinity purification | |
| Isotype: | IgG |
Background
The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells.
Immunogen Information
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 304-473 of human CAMK4 (NP_001735.1). |
| GeneID: | 814 |
| SwissProt: | Q16566 |
| Synonyms: | CAMK4; CaMK IV; CaMK-GR; caMK; CaMKIV; calcium/calmodulin dependent protein kinase IV |
| Calculated MW: | 51kDa |
| Observed MW: | 58kDa |
Applications
| Reactivity: | Human, Mouse, Rat |
| Tested Applications: | WB; IF |
| Recommended Dilution: | WB 1:500 - 1:2000; IF 1:50 - 1:200 |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Application Key: | Western blotting Immunofluorescence |
| Positive Samples: | SW620, U-87MG, Mouse brain, Mouse thymus |
| Cellular Location: | Cytoplasm, Nucleus |
Antibody Images
| Western blot analysis of extracts of various cell lines, using CAMK4 antibody (CAB5304) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s. |
| Immunofluorescence analysis of U2OS cells using CAMK4 antibody (CAB5304). Blue: DAPI for nuclear staining. |
Datasheets
Additional Information
Antibody Type: |
Polyclonal |
Application: |
WB |
Application: |
IF |
Reactivity: |
Human |
Reactivity: |
Mouse |
Reactivity: |
Rat |
Host Species: |
Rabbit |
Isotype: |
IgG |
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