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C9orf72 Polyclonal Antibody (CAB15970)

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SKU:
CAB15970
$331.89

Description

Overview

Title:C9orf72 Polyclonal Antibody (CAB15970)
Size:100µL
Code:CAB15970
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene plays an important role in the regulation of endosomal trafficking, and has been shown to interact with Rab proteins that are involved in autophagy and endocytic transport. Expansion of a GGGGCC repeat from 2-22 copies to 700-1600 copies in the intronic sequence between alternate 5' exons in transcripts from this gene is associated with 9p-linked ALS (amyotrophic lateral sclerosis) and FTD (frontotemporal dementia) (PMID: 21944778, 21944779). Studies suggest that hexanucleotide expansions could result in the selective stabilization of repeat-containing pre-mRNA, and the accumulation of insoluble dipeptide repeat protein aggregates that could be pathogenic in FTD-ALS patients (PMID: 23393093). Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human C9orf72 (NP_060795.1).
GeneID:203228
SwissProt:Q96LT7
Synonyms:C9orf72; ALSFTD; DENNL72; FTDALS; FTDALS1; protein C9orf72
Calculated MW:24kDa/54kDa
Observed MW:54kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:Mouse kidney
Cellular Location:Cytoplasm, Cytoplasmic vesicle, Endosome, Lysosome, Nucleus, Secreted, autophagosome

Antibody Images

Western blot - C9orf72 Polyclonal Antibody (CAB15970)

Western blot analysis of extracts of mouse kidney, using C9orf72 antibody (CAB15970) at 1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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