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BUB3 Polyclonal Antibody (CAB6536)

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CAB6536
  • Western blot - BUB3 Polyclonal Antibody (CAB6536)
  • Immunofluorescence - BUB3 Polyclonal Antibody (CAB6536)
€299.00

Description

Overview

Title:BUB3 Polyclonal Antibody (CAB6536)
Size:100µL
Code:CAB6536
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human BUB3 (NP_004716.1).
GeneID:9184
SwissProt:O43684
Synonyms:BUB3; BUB3L; hBUB3; mitotic checkpoint protein BUB3
Calculated MW:36kDa/37kDa
Observed MW:40kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IF
Recommended Dilution:WB 1:500 - 1:2000; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:MCF-7, HL-60, HeLa, Mouse spleen, Mouse ovary, Mouse brain, Mouse lung
Cellular Location:Chromosome, Nucleus, centromere, kinetochore

Antibody Images

Western blot - BUB3 Polyclonal Antibody (CAB6536)

Western blot analysis of extracts of various cell lines, using BUB3 antibody (CAB6536) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 90s.

Immunofluorescence - BUB3 Polyclonal Antibody (CAB6536)

Immunofluorescence analysis of U2OS cells using BUB3 antibody (CAB6536).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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