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BRCC3 Polyclonal Antibody (CAB4965)

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SKU:
CAB4965
€299.00

Description

Overview

Title:BRCC3 Polyclonal Antibody (CAB4965)
Size:100µL
Code:CAB4965
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. This complex plays a role in the DNA damage response, where it is responsible for the stable accumulation of BRCA1 at DNA break sites. The component encoded by this gene can specifically cleave Lys 63-linked polyubiquitin chains, and it regulates the abundance of these polyubiquitin chains in chromatin. The loss of this gene results in abnormal angiogenesis and is associated with syndromic moyamoya, a cerebrovascular angiopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 5.

Immunogen Information

Immunogen:A synthetic Peptide of human BRCC3
GeneID:79184
SwissProt:P46736
Synonyms:BRCC3; BRCC36; C6.1A; CXorf53; lys-63-specific deubiquitinase BRCC36
Calculated MW:23kDa/28kDa/33kDa/36kDa
Observed MW:36kDa

Applications

Reactivity:Human
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:1000
Storage Buffer:Store at 4°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, pH7.3.
Application Key:Western blotting    
Positive Samples:MCF7
Cellular Location:Nucleus

Antibody Images

Western blot - BRCC3 Polyclonal Antibody (CAB4965)

Western blot analysis of extracts of MCF-7 cells, using BRCC3 antibody (CAB4965).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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