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BRCA1 Polyclonal Antibody (CAB11034)

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CAB11034
  • Western blot - BRCA1 Polyclonal Antibody (CAB11034)
  • Immunofluorescence - BRCA1 Polyclonal Antibody (CAB11034)
€299.00

Description

Overview

Title:BRCA1 Polyclonal Antibody (CAB11034)
Size:100µL
Code:CAB11034
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 400-699 of human BRCA1 (NP_009230.2).
GeneID:672
SwissProt:P38398
Synonyms:BRCA1; BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4; PPP1R53; PSCP; RNF53; BRCA1, DNA repair associated
Calculated MW:7kDa/78-85kDa/202-210kDa
Observed MW:280kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IF
Recommended Dilution:WB 1:500 - 1:2000; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:HL-60, HeLa, K-562, Mouse liver
Cellular Location:Chromosome, Cytoplasm, Cytoplasm, Nucleus

Antibody Images

Western blot - BRCA1 Polyclonal Antibody (CAB11034)

Western blot analysis of extracts of various cell lines, using BRCA1 antibody (CAB11034) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.

Immunofluorescence - BRCA1 Polyclonal Antibody (CAB11034)

Immunofluorescence analysis of HeLa cells using BRCA1 antibody (CAB11034) at dilution of 1:100. Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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