BICD2 Polyclonal Antibody (CAB10292)

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CAB10292
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Description

Antibody DatasheetAntibody MSDS

BICD2 Polyclonal Antibody (CAB10292)

Overview

Title:BICD2 Polyclonal Antibody (CAB10292)
Size:100µL
Code:CAB10292
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene is one of two human homologs of Drosophila bicaudal-D and a member of the Bicoid family. It has been implicated in dynein-mediated, minus end-directed motility along microtubules. It has also been reported to be a phosphorylation target of NIMA related kinase 8. Two alternative splice variants have been described.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 440-620 of human BICD2 (NP_001003800.1).
GeneID:23299
SwissProt:Q8TD16
Synonyms:BICD2; SMALED2; bA526D8.1; BICD cargo adaptor 2
Calculated MW:93kDa/96kDa
Observed MW:118kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB
Recommended Dilution:WB 1:1000 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:U-87MG, HL-60, HeLa, Mouse brain, Rat brain
Cellular Location:Cytoplasm, Golgi apparatus, cytoskeleton

Antibody Images

Western blot - BICD2 Polyclonal Antibody (CAB10292)

Western blot analysis of extracts of various cell lines, using BICD2 antibody (CAB10292) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

BICD2 Polyclonal Antibody (CAB10292) Protein Information

UniProt Protein Function:BICD2: May play a role in the dynein-dynactin interactions on the surface of membranous organelles, by associating with these complexes. Regulates coat complex coatomer protein I (COPI)- independent Golgi-endoplasmic reticulum transport by recruiting the dynein-dynactin motor complex. Belongs to the BicD family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 9q22.31

Cellular Component: annulate lamellae; cytoplasmic vesicle; cytosol; Golgi apparatus; nuclear envelope; nuclear pore; plasma membrane

Molecular Function:protein binding; Rab GTPase binding

Biological Process: centrosome localization; protein targeting to Golgi; retrograde vesicle-mediated transport, Golgi to ER

Disease: Spinal Muscular Atrophy, Lower Extremity-predominant, 2, Autosomal Dominant

NCBI Summary:This gene is one of two human homologs of Drosophila bicaudal-D and a member of the Bicoid family. It has been implicated in dynein-mediated, minus end-directed motility along microtubules. It has also been reported to be a phosphorylation target of NIMA related kinase 8. Two alternative splice variants have been described. [provided by RefSeq, Jul 2008]
UniProt Code:Q8TD16
NCBI GenInfo Identifier:34098604
NCBI Gene ID:23299
NCBI Accession:Q8TD16.1
UniProt Secondary Accession:Q8TD16,O75181, Q5TBQ2, Q5TBQ3, Q96LH2, Q9BT84, Q9H561
UniProt Related Accession:Q8TD16
Molecular Weight:94kDa
NCBI Full Name:Protein bicaudal D homolog 2
NCBI Synonym Full Names:BICD cargo adaptor 2
NCBI Official Symbol:BICD2  
NCBI Official Synonym Symbols:SMALED2; bA526D8.1  
NCBI Protein Information:protein bicaudal D homolog 2
UniProt Protein Name:Protein bicaudal D homolog 2
Protein Family:Protein bicaudal
UniProt Gene Name:BICD2  
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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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