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Beta-tubulin Polyclonal Antibody (CABC015)

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SKU:
CABC015
  • Western blot - ?-tubulin Polyclonal Antibody (CABC015)
  • Immunofluorescence - ?-tubulin Polyclonal Antibody (CABC015)
  • Immunohistochemistry - ?-tubulin Polyclonal Antibody (CABC015)
  • Immunohistochemistry - ?-tubulin Polyclonal Antibody (CABC015)
  • Immunohistochemistry - ?-tubulin Polyclonal Antibody (CABC015)
$298.59

Description

Overview

Title:Beta-tubulin Polyclonal Antibody (CABC015)
Size:100uL
Code:CABC015
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human ?-tubulin (NP_006077.2).
GeneID:10381
SwissProt:P07437
Synonyms:TUBB3; CDCBM; CDCBM1; CFEOM3; CFEOM3A; FEOM3; TUBB4; beta-4; CDCBM6; CSCSC1; M40; OK/SW-cl.56; TUBB1; TUBB5; tubulin beta-3 chain
Calculated MW:49kDa
Observed MW:50kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF;
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:SH-SY5Y, HeLa, A-549, MCF7, HepG2, Mouse eye, Rat spinal cord
Cellular Location:Cytoplasm, cytoskeleton

Antibody Images

Western blot - Beta-tubulin Polyclonal Antibody (CABC015)

Western blot analysis of extracts of various cell lines, using TUBB antibody (CABC015) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.

Immunohistochemistry - Beta-tubulin Polyclonal Antibody (CABC015)

Immunohistochemistry of paraffin-embedded HepG2 cells using Beta-Tubulin antibody (CABC015) at dilution of 1:100 (40x lens).

Immunohistochemistry - Beta-tubulin Polyclonal Antibody (CABC015)

Immunohistochemistry of paraffin-embedded HepG2 cells treated by oleic acid using Beta-Tubulin antibody (CABC015) at dilution of 1:100 (40x lens).

Immunohistochemistry - Beta-tubulin Polyclonal Antibody (CABC015)

Immunohistochemistry of paraffin-embedded HepG2 cells treated by oleic acid using Beta-Tubulin antibody (CABC015) at dilution of 1:100 (40x lens).

Immunofluorescence - Beta-tubulin Polyclonal Antibody (CABC015)

Immunofluorescence analysis of HeLa cells using TUBB antibody (CABC015) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Additional Information

ANTIBODY TYPE:
Polyclonal
APPLICATION:
WB
APPLICATION:
IHC
APPLICATION:
IF
REACTIVITY:
Human
REACTIVITY:
Mouse
REACTIVITY:
Rat
HOST SPECIES:
Rabbit
ISOTYPE:
IgG
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