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Beta-Tubulin Polyclonal Antibody (CABC008)

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CABC008
  • Western blot - ?-Tubulin Polyclonal Antibody (CABC008)
  • Western blot - ?-Tubulin Polyclonal Antibody (CABC008)
$298.59

Description

Overview

Title:Beta-Tubulin Polyclonal Antibody (CABC008)
Size:100uL
Code:CABC008
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human ?-Tubulin (NP_006077.2).
GeneID:203068
SwissProt:P07437
Synonyms:TUBB; CDCBM6; CSCSC1; M40; OK/SW-cl.56; TUBB1; TUBB5; tubulin beta chain
Calculated MW:50kDa
Observed MW:50kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC;
Recommended Dilution:WB 1:500 - 1:5000; IHC 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:BT474, HeLa, U251, MCF7, mouse brain, mouse spinal cord, rat brain, rat spinal cord, HeLa
Cellular Location:Cytoplasm, cytoskeleton

Antibody Images

Western blot - Beta-Tubulin Polyclonal Antibody (CABC008)

Western blot analysis of extracts of various cell lines, using Beta-Tubulin antibody (CABC008) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - Beta-Tubulin Polyclonal Antibody (CABC008)

Western blot analysis of HeLa cell using Beta-tubulin antibody (CABC008) at different dilution. Each lane was loaded with 10 ug cell lysate.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
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Additional Information

ANTIBODY TYPE:
Polyclonal
APPLICATION:
WB
APPLICATION:
IHC
REACTIVITY:
Human
REACTIVITY:
Mouse
REACTIVITY:
Rat
HOST SPECIES:
Rabbit
ISOTYPE:
IgG
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