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Beta Actin Monoclonal Antibody (CABC026)

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CABC026
  • Western blot - ACTB Monoclonal Antibody (CABC026)
  • Immunofluorescence - ACTB Monoclonal Antibody (CABC026)
  • Immunohistochemistry - ACTB Monoclonal Antibody (CABC026)
  • Immunohistochemistry - ACTB Monoclonal Antibody (CABC026)
  • Immunohistochemistry - ACTB Monoclonal Antibody (CABC026)
  • Western blot - ACTB Monoclonal Antibody (CABC026)
$331.89

Description

Overview
Title: ACTB Monoclonal Antibody (CABC026)
Size: 100uL
Code: CABC026
Host Species: Rabbit
Purification: Affinity purification
Isotype: IgG  
BackgroundThis gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. |Put 5ul AC026 into 45 ul buffer(with 50% glycerol), diluted 1:10, 000 for using. The diluted antibody can be stored at -20'C without aliquot.Immunogen Information
Immunogen: Recombinant protein of human Beta-actin
GeneID: 60
SwissProt: P60709
Synonyms: ACTB; BRWS1; PS1TP5BP1; beta actin
Calculated MW: 41kDa
Observed MW: 43kDa
Applications
Reactivity: Human, Mouse, Rat, Chicken, Zebrafish, Pig, Oryza sativa
Tested Applications: WB; IHC; IF;
Recommended Dilution: WB 1:50000 - 1:200000; IHC 1:200 - 1:2000; IF 1:200 - 1:2000
Storage Buffer: Store at -20'C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key: Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples: NIH/3T3, TE-1, 293T, SW480, Mouse brain, Mouse testis, Mouse lung, Rat brain, HeLa
Cellular Location: Cytoplasm, cytoskeleton
Antibody Images

Western blot - ACTB Monoclonal Antibody (CABC026)

Western blot analysis of extracts of various cell lines, using Beta-actin antibody (CABC026) at 1:100000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Western blot - ACTB Monoclonal Antibody (CABC026)

Western blot analysis of extracts of HeLa cells, using Beta-actin antibody (CABC026) at 1:10000-1:2560000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

Immunohistochemistry - ACTB Monoclonal Antibody (CABC026)

Immunohistochemistry of paraffin-embedded human breast cancer using Beta-actin antibody (CABC026) at dilution of 1:100 (20x lens).

Immunohistochemistry - ACTB Monoclonal Antibody (CABC026)

Immunohistochemistry of paraffin-embedded mouse liver using Beta-actin antibody (CABC026) at dilution of 1:100 (20x lens).

Immunohistochemistry - ACTB Monoclonal Antibody (CABC026)

Immunohistochemistry of paraffin-embedded rat kidney using Beta-actin antibody (CABC026) at dilution of 1:100 (20x lens).

Immunofluorescence - ACTB Monoclonal Antibody (CABC026)

Immunofluorescence analysis of HeLa cells using ACTB antibody (CABC026) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Additional Information

ANTIBODY TYPE:
Monoclonal
APPLICATION:
WB
APPLICATION:
IHC
APPLICATION:
IF
REACTIVITY:
Human
REACTIVITY:
Mouse
REACTIVITY:
Rat
REACTIVITY:
Chicken
REACTIVITY:
Zebrafish
REACTIVITY:
Pig
REACTIVITY:
Oryza sativa
HOST SPECIES:
Rabbit
ISOTYPE:
IgG
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