BCR Polyclonal Antibody (CAB0068)

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CAB0068
  • Western blot - BCR Polyclonal Antibody (CAB0068)
  • Immunohistochemistry - BCR Polyclonal Antibody (CAB0068)
  • Immunohistochemistry - BCR Polyclonal Antibody (CAB0068)
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Description

Antibody DatasheetAntibody MSDS

BCR Polyclonal Antibody (CAB0068)

Overview

Title:BCR Polyclonal Antibody (CAB0068)
Size:100µL
Code:CAB0068
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human BCR (NP_004318.3).
GeneID:613
SwissProt:P11274
Synonyms:BCR; ALL; BCR1; CML; D22S11; D22S662; PHL; breakpoint cluster region protein
Calculated MW:137kDa/142kDa
Observed MW:150kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:293T, Jurkat, HeLa, Mouse brain, Rat brain
Cellular Location:Cell junction, postsynaptic cell membrane, postsynaptic density, synapse

Antibody Images

Western blot - BCR Polyclonal Antibody (CAB0068)

Western blot analysis of extracts of various cell lines, using BCR antibody (CAB0068) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Immunohistochemistry - BCR Polyclonal Antibody (CAB0068)

Immunohistochemistry of paraffin-embedded human stomach using BCR antibody (CAB0068) at dilution of 1:100 (40x lens).

Immunohistochemistry - BCR Polyclonal Antibody (CAB0068)

Immunohistochemistry of paraffin-embedded human prostate using BCR antibody (CAB0068) at dilution of 1:100 (40x lens).

BCR Polyclonal Antibody (CAB0068) Protein Information

UniProt Protein Function:Bcr: a protein with serine/threonine protein kinase activity and is a GTPase-activating protein (GAP) for RAC1 and CDC42. The amino-terminal region of Bcr contains an oligomerization domain, a serine/threonine kinase domain and a region that binds SH2 domains. The middle of the protein has a PH domain and a region of sequence similarity to the guanine nucleotide exchange factors for the Rho family of GTP binding proteins. The carboxy-terminal region promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. A breakpoint cluster region protein that participates in a t(9;22)(q34;q11) chromosomal translocation that produces a BCR-ABL oncogene responsible for chronic myeloid leukemia (CML), acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). The function of wild type Bcr in cells remains unclear. PDGF receptor may use Bcr as a downstream signaling mediator. Tyr177 of human Bcr, phosphorylated in the Bcr-Abl fusion protein, provides a docking site for Gab2 and GRB2, and is important in the transforming activity of Bcr-Abl. Sequence variants of the Bcr protein may be associated with bipolar disorder. Two alternatively spliced isoforms of the human protein have been reported.
UniProt Protein Details:

Protein type:Kinase, protein; GAPs; Oncoprotein; GEFs, Rac/Rho; Protein kinase, atypical; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; GAPs, Rac/Rho; ATYPICAL group; BCR family

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: postsynaptic membrane; protein complex; membrane; postsynaptic density; cytosol; cell junction

Molecular Function:protein serine/threonine kinase activity; protein binding; Rho guanyl-nucleotide exchange factor activity; enzyme binding; protein-tyrosine kinase activity; kinase activity; ATP binding; GTPase activator activity

Biological Process: inner ear morphogenesis; peptidyl-tyrosine phosphorylation; regulation of cell cycle; protein amino acid autophosphorylation; platelet-derived growth factor receptor signaling pathway; response to lipopolysaccharide; signal transduction; protein amino acid phosphorylation; regulation of small GTPase mediated signal transduction; negative regulation of neutrophil degranulation; negative regulation of inflammatory response; small GTPase mediated signal transduction; positive regulation of phagocytosis; brain development; neuromuscular process controlling balance; actin cytoskeleton organization and biogenesis; negative regulation of cell migration

Disease: Leukemia, Acute Lymphoblastic; Leukemia, Chronic Myeloid

NCBI Summary:A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P11274
NCBI GenInfo Identifier:143811366
NCBI Gene ID:613
NCBI Accession:P11274.2
UniProt Secondary Accession:P11274,P78501, Q12842, Q4LE80, Q6NZI3,
UniProt Related Accession:P11274
Molecular Weight:1271
NCBI Full Name:Breakpoint cluster region protein
NCBI Synonym Full Names:breakpoint cluster region
NCBI Official Symbol:BCR  
NCBI Official Synonym Symbols:ALL; CML; PHL; BCR1; D22S11; D22S662  
NCBI Protein Information:breakpoint cluster region protein; BCR/FGFR1 chimera protein; FGFR1/BCR chimera protein; renal carcinoma antigen NY-REN-26
UniProt Protein Name:Breakpoint cluster region protein
UniProt Synonym Protein Names:Renal carcinoma antigen NY-REN-26
Protein Family:Bicyclomycin resistance protein
UniProt Gene Name:BCR  
UniProt Entry Name:BCR_HUMAN
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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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