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BCL2 Polyclonal Antibody (CAB2212)

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SKU:
CAB2212
$476.19

Description

Overview

Title:BCL2 Polyclonal Antibody (CAB2212)
Size:100µL
Code:CAB2212
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.

Immunogen Information

Immunogen:Recombinant protein of human BCL2
GeneID:596
SwissProt:P10415
Synonyms:BCL2; Bcl-2; PPP1R50; apoptosis regulator Bcl-2
Calculated MW:22kDa/26kDa
Observed MW:25kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IP; FC
Recommended Dilution:WB 1:500 - 1:1000; IHC 1:50 - 1:100; IP 1:20 - 1:50; FC 1:20 - 1:50
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunoprecipitation    Flow Cytometry    
Positive Samples:Jurkat, U-937, MCF-7, Mouse liver, Rat brain
Cellular Location:Endoplasmic reticulum membrane, Mitochondrion outer membrane, Nucleus membrane, Single-pass membrane protein

Antibody Images

Western blot - BCL2 Polyclonal Antibody (CAB2212)

Western blot analysis of extracts of various cell lines, using BCL2 antibody (CAB2212) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IP
Application:
FC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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