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BCL2 Polyclonal Antibody (CAB16776)

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CAB16776
  • Western blot - BCL2 Polyclonal Antibody (CAB16776)
  • Immunohistochemistry - BCL2 Polyclonal Antibody (CAB16776)
  • Immunohistochemistry - BCL2 Polyclonal Antibody (CAB16776)
  • Immunohistochemistry - BCL2 Polyclonal Antibody (CAB16776)
  • Immunohistochemistry - BCL2 Polyclonal Antibody (CAB16776)
  • Immunofluorescence - BCL2 Polyclonal Antibody (CAB16776)
  • Immunofluorescence - BCL2 Polyclonal Antibody (CAB16776)
  • Immunofluorescence - BCL2 Polyclonal Antibody (CAB16776)
€299.00

Description

Overview

Title:BCL2 Polyclonal Antibody (CAB16776)
Size:100µL
Code:CAB16776
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.

Immunogen Information

Immunogen:A synthetic peptide of human BCL2.
GeneID:596
SwissProt:P10415
Synonyms:BCL2; Bcl-2; PPP1R50; apoptosis regulator Bcl-2
Calculated MW:22kDa/26kDa
Observed MW:26kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:293T, HeLa, mouse lung, mouse testis
Cellular Location:N/A

Antibody Images

Western blot - BCL2 Polyclonal Antibody (CAB16776)

Western blot analysis of extracts of various cells, using BCL2 antibody (CAB16776) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

Immunohistochemistry - BCL2 Polyclonal Antibody (CAB16776)

Immunohistochemistry of paraffin-embedded human liver cancer using BCL2 antibody (CAB16776) at dilution of 1:200 (40x lens).

Immunohistochemistry - BCL2 Polyclonal Antibody (CAB16776)

Immunohistochemistry of paraffin-embedded human colon carcinoma using BCL2 antibody (CAB16776) at dilution of 1:200 (40x lens).

Immunohistochemistry - BCL2 Polyclonal Antibody (CAB16776)

Immunohistochemistry of paraffin-embedded human breast cancer using BCL2 antibody (CAB16776) at dilution of 1:200 (40x lens).

Immunohistochemistry - BCL2 Polyclonal Antibody (CAB16776)

Immunohistochemistry of paraffin-embedded human stomach using BCL2 antibody (CAB16776) at dilution of 1:200 (40x lens).

Immunofluorescence - BCL2 Polyclonal Antibody (CAB16776)

Immunofluorescence analysis of C6 cells using BCL2 antibody (CAB16776) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence - BCL2 Polyclonal Antibody (CAB16776)

Immunofluorescence analysis of NIH/3T3 cells using BCL2 antibody (CAB16776) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence - BCL2 Polyclonal Antibody (CAB16776)

Immunofluorescence analysis of U2OS cells using BCL2 antibody (CAB16776) at dilution of 1:100. Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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