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BAX Polyclonal Antibody (CAB11550)

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CAB11550
  • Western blot - [KO Validated] BAX Polyclonal Antibody (CAB11550)
  • Western blot - [KO Validated] BAX Polyclonal Antibody (CAB11550)
  • Immunofluorescence - [KO Validated] BAX Polyclonal Antibody (CAB11550)
  • Immunofluorescence - [KO Validated] BAX Polyclonal Antibody (CAB11550)
  • Immunofluorescence - [KO Validated] BAX Polyclonal Antibody (CAB11550)
  • Immunofluorescence - [KO Validated] BAX Polyclonal Antibody (CAB11550)
$331.89

Description

Overview

Title:BAX Polyclonal Antibody (CAB11550)
Size:100µL
Code:CAB11550
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human BAX (NP_620116.1).
GeneID:581
SwissProt:Q07812
Synonyms:BAX; BCL2L4; apoptosis regulator BAX
Calculated MW:4kDa/12kDa/15kDa/18kDa/19kDa/21kDa/24kDa
Observed MW:23kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:1000; IHC 1:50 - 1:100; IF 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:MCF-7, HT-1080
Cellular Location:Cytoplasm, Cytoplasm, Mitochondrion membrane, Single-pass membrane protein

Antibody Images

Western blot - [KO Validated] BAX Polyclonal Antibody (CAB11550)

Western blot analysis of extracts of various cell lines, using BAX antibody (CAB11550) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

Western blot - [KO Validated] BAX Polyclonal Antibody (CAB11550)

Western blot analysis of extracts from normal (control) and BAX knockout (KO) 293T cells, using BAX antibody (CAB11550) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Immunofluorescence - [KO Validated] BAX Polyclonal Antibody (CAB11550)

Immunofluorescence analysis of C6 cells using BAX antibody (CAB11550) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence - [KO Validated] BAX Polyclonal Antibody (CAB11550)

Immunofluorescence analysis of NIH/3T3 cells using BAX antibody (CAB11550) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence - [KO Validated] BAX Polyclonal Antibody (CAB11550)

Immunofluorescence analysis of U2OS cells using BAX antibody (CAB11550) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence - [KO Validated] BAX Polyclonal Antibody (CAB11550)

Immunofluorescence analysis of HeLa cells using BAX antibody (CAB11550) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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