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BAX Monoclonal Antibody (CAB2211)

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SKU:
CAB2211
$476.19

Description

Overview

Title:BAX Monoclonal Antibody (CAB2211)
Size:100µL
Code:CAB2211
Host Species:Mouse
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.

Immunogen Information

Immunogen:Recombinant protein of human BAX
GeneID:581
SwissProt:Q07812
Synonyms:BAX; BCL2L4; apoptosis regulator BAX
Calculated MW:4kDa/12kDa/15kDa/18kDa/19kDa/21kDa/24kDa
Observed MW:21kDa

Applications

Reactivity:Human, Monkey
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:1000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:COS7, Raji, HeLa
Cellular Location:Cytoplasm, Cytoplasm, Mitochondrion membrane, Single-pass membrane protein

Antibody Images

Western blot - BAX Monoclonal Antibody (CAB2211)

Western blot analysis of extracts of various cell lines, using BAX antibody (CAB2211) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Monoclonal
Application:
WB
Reactivity:
Human
Reactivity:
Monkey
Host Species:
Mouse
Isotype:
IgG
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