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BANF1 Polyclonal Antibody (CAB14555)

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SKU:
CAB14555
$331.89

Description

Overview

Title:BANF1 Polyclonal Antibody (CAB14555)
Size:100µL
Code:CAB14555
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene was first identified by its ability to protect retroviruses from intramolecular integration and therefore promote intermolecular integration into the host cell genome. The protein forms a homodimer which localizes to both the nucleus and cytoplasm and is specifically associated with chromosomes during mitosis. This protein binds to double stranded DNA in a non-specific manner and also binds to LEM-domain containing proteins of the nuclear envelope. This protein is thought to facilitate nuclear reassembly by binding with both DNA and inner nuclear membrane proteins and thereby recruit chromatin to the nuclear periphery. Alternative splicing results in multiple transcript variants encoding the same protein.

Immunogen Information

Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-89 of human BANF1 (NP_001137457.1).
GeneID:8815
SwissProt:O75531
Synonyms:BANF1; BAF; BCRP1; D14S1460; NGPS; barrier-to-autointegration factor
Calculated MW:10kDa
Observed MW:10kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:SW480, 293T, Mouse thymus, Rat brain
Cellular Location:Chromosome, Cytoplasm, Nucleus

Antibody Images

Western blot - BANF1 Polyclonal Antibody (CAB14555)

Western blot analysis of extracts of various cell lines, using BANF1 antibody (CAB14555) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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