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BAIAP2L1 Polyclonal Antibody (CAB8238)

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SKU:
CAB8238
$331.89

Description

Overview

Title:BAIAP2L1 Polyclonal Antibody (CAB8238)
Size:100µL
Code:CAB8238
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the IMD (IRSp53/MIM homology domain) family. Members of this family can be subdivided in two groups, the IRSp53-like and MIM-like, based on the presence or absence of the SH3 (Src homology 3) domain. The protein encoded by this gene contains a conserved IMD, also known as F-actin bundling domain, at the N-terminus, and a canonical SH3 domain near the C-terminus, so it belongs to the IRSp53-like group. This protein is the substrate for insulin receptor tyrosine kinase and binds to the small GTPase Rac. It is involved in signal transduction pathways that link deformation of the plasma membrane and remodeling of the actin cytoskeleton. It also promotes actin assembly and membrane protrusions when overexpressed in mammalian cells, and is essential to the formation of a potent actin assembly complex during EHEC (Enterohemorrhagic Escherichia coli) pedestal formation.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 198-388 of human BAIAP2L1 (NP_061330.2).
GeneID:55971
SwissProt:Q9UHR4
Synonyms:BAIAP2L1; IRTKS; BAI1 associated protein 2 like 1
Calculated MW:56kDa
Observed MW:70kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:HepG2, HeLa, 293T, A-549, Mouse lung
Cellular Location:Cytoplasm, cytoskeleton

Antibody Images

Western blot - BAIAP2L1 Polyclonal Antibody (CAB8238)

Western blot analysis of extracts of various cell lines, using BAIAP2L1 antibody (CAB8238) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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