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AXIN1 Polyclonal Antibody (CAB16019)

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SKU:
CAB16019
$331.89

Description

Overview

Title:AXIN1 Polyclonal Antibody (CAB16019)
Size:100µL
Code:CAB16019
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Alternative splicing results in multiple transcript variants.

Immunogen Information

Immunogen:Recombinant protein of human AXIN1
GeneID:8312
SwissProt:O15169
Synonyms:AXIN1; AXIN; PPP1R49; axin-1
Calculated MW:91kDa/95kDa
Observed MW:110kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:1000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:A-549, mouse kidney, rat thymus
Cellular Location:Cell membrane, Cytoplasm, Membrane, Nucleus

Antibody Images

Western blot - AXIN1 Polyclonal Antibody (CAB16019)

Western blot analysis of extracts of various cell lines, using AXIN1 antibody (CAB16019) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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