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ATG9B Polyclonal Antibody (CAB7406)

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SKU:
CAB7406
€299.00

Description

Overview

Title:ATG9B Polyclonal Antibody (CAB7406)
Size:100µL
Code:CAB7406
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene functions in the regulation of autophagy, a lysosomal degradation pathway. This gene also functions as an antisense transcript in the posttranscriptional regulation of the endothelial nitric oxide synthase 3 gene, which has 3' overlap with this gene on the opposite strand. Mutations in this gene and disruption of the autophagy process have been associated with multiple cancers. Alternative splicing results in multiple transcript variants.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 715-924 of human ATG9B (NP_775952.4).
GeneID:285973
SwissProt:Q674R7
Synonyms:ATG9B; APG9L2; NOS3AS; SONE; autophagy related 9B
Calculated MW:36kDa/44kDa/101kDa
Observed MW:101kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:HepG2, HL-60, Mouse liver, Mouse brain, Rat brain
Cellular Location:Cytoplasmic vesicle, Multi-pass membrane protein, autophagosome membrane

Antibody Images

Western blot - ATG9B Polyclonal Antibody (CAB7406)

Western blot analysis of extracts of various cell lines, using ATG9B antibody (CAB7406) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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