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ATG7 Polyclonal Antibody (CAB0691)

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CAB0691
  • Western blot - ATG7 Polyclonal Antibody (CAB0691)
  • Immunohistochemistry - ATG7 Polyclonal Antibody (CAB0691)
  • Immunohistochemistry - ATG7 Polyclonal Antibody (CAB0691)
  • Immunohistochemistry - ATG7 Polyclonal Antibody (CAB0691)
$331.89

Description

Overview

Title:ATG7 Polyclonal Antibody (CAB0691)
Size:100µL
Code:CAB0691
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 500-676 of human ATG7 (NP_001129503.2).
GeneID:10533
SwissProt:O95352
Synonyms:ATG7; APG7-LIKE; APG7L; GSA7; autophagy related 7
Calculated MW:68kDa/75kDa/77kDa
Observed MW:78kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:Jurkat, THP-1, HeLa, 293T, Mouse kidney, Mouse liver, Rat kidney, Rat liver
Cellular Location:Cytoplasm, Preautophagosomal structure

Antibody Images

Western blot - ATG7 Polyclonal Antibody (CAB0691)

Western blot analysis of extracts of various cell lines, using ATG7 antibody (CAB0691) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

Immunohistochemistry - ATG7 Polyclonal Antibody (CAB0691)

Immunohistochemistry of paraffin-embedded rat brain using ATG7 antibody (CAB0691) at dilution of 1:100 (40x lens).

Immunohistochemistry - ATG7 Polyclonal Antibody (CAB0691)

Immunohistochemistry of paraffin-embedded mouse brain using ATG7 antibody (CAB0691) at dilution of 1:100 (40x lens).

Immunohistochemistry - ATG7 Polyclonal Antibody (CAB0691)

Immunohistochemistry of paraffin-embedded human tonsil using ATG7 antibody (CAB0691) at dilution of 1:100 (40x lens).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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