ATG5 Polyclonal Antibody (CAB7252)

Autophagy protein 5 Polyclonal Antibody (CAB7252)

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CAB7252

Description
Additional Information

Description

Autophagy protein 5 Polyclonal Antibody (CAB7252)

Overview

Title:	ATG5 Polyclonal Antibody (CAB7252)
Size:	100µL
Code:	CAB7252
Host Species:	Rabbit
Purification:	Affinity purification
Isotype:	IgG

Background

The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene.

Immunogen Information

Immunogen:	A synthetic peptide of human ATG5
GeneID:	9474
SwissProt:	Q9H1Y0
Synonyms:	ATG5; APG5; APG5-LIKE; APG5L; ASP; hAPG5; autophagy protein 5
Calculated MW:	22kDa/32kDa
Observed MW:	54kDa

Applications

Reactivity:	Human, Mouse
Tested Applications:	WB; IHC; IF
Recommended Dilution:	WB 1:500 - 1:2000; IHC 1:10 - 1:100; IF 1:10 - 1:100
Storage Buffer:	Store at 4°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, pH7.3.
Application Key:	Western blotting Immunohistochemistry Immunofluorescence
Positive Samples:	Mouse kidney
Cellular Location:	Cytoplasm, Peripheral membrane protein, Preautophagosomal structure membrane

Antibody Images

Western blot analysis of extracts of mouse kidney, using ATG5 antibody (CAB7252).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Autophagy protein 5 Polyclonal Antibody (CAB7252) Protein Information

UniProt Code:	Q9H1Y0
NCBI GenInfo Identifier:	17366828
NCBI Gene ID:	9474
NCBI Accession:	Q9H1Y0.2
UniProt Secondary Accession:	Q9H1Y0,O60875, Q5JVR2, Q68DI4, Q9H2B8, Q9HCZ7,
UniProt Related Accession:	Q9H1Y0
Molecular Weight:	275
NCBI Full Name:	Autophagy protein 5
NCBI Synonym Full Names:	autophagy related 5
NCBI Official Symbol:	ATG5
NCBI Official Synonym Symbols:	ASP; APG5; APG5L; hAPG5; APG5-LIKE
NCBI Protein Information:	autophagy protein 5; apoptosis-specific protein; ATG5 autophagy related 5 homolog
UniProt Protein Name:	Autophagy protein 5
UniProt Synonym Protein Names:	APG5-like; Apoptosis-specific protein
Protein Family:	Autophagy protein
UniProt Gene Name:	ATG5
UniProt Entry Name:	ATG5_HUMAN