ATG5 Polyclonal Antibody (CAB7252)
- SKU:
- CAB7252
Description
| Title: | ATG5 Polyclonal Antibody (CAB7252) | |
| Size: | 100µL | |
| Code: | CAB7252 | |
| Host Species: | Rabbit | |
| Purification: | Affinity purification | |
| Isotype: | IgG |
Background
The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene.
Immunogen Information
| Immunogen: | A synthetic peptide of human ATG5 |
| GeneID: | 9474 |
| SwissProt: | Q9H1Y0 |
| Synonyms: | ATG5; APG5; APG5-LIKE; APG5L; ASP; hAPG5; autophagy protein 5 |
| Calculated MW: | 22kDa/32kDa |
| Observed MW: | 54kDa |
Applications
| Reactivity: | Human, Mouse |
| Tested Applications: | WB; IHC; IF |
| Recommended Dilution: | WB 1:500 - 1:2000; IHC 1:10 - 1:100; IF 1:10 - 1:100 |
| Storage Buffer: | Store at 4°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, pH7.3. |
| Application Key: | Western blotting Immunohistochemistry Immunofluorescence |
| Positive Samples: | Mouse kidney |
| Cellular Location: | Cytoplasm, Peripheral membrane protein, Preautophagosomal structure membrane |
Antibody Images
| Western blot analysis of extracts of mouse kidney, using ATG5 antibody (CAB7252).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. |
Datasheets
Additional Information
Antibody Type: |
Polyclonal |
Application: |
WB |
Application: |
IHC |
Application: |
IF |
Reactivity: |
Human |
Reactivity: |
Mouse |
Host Species: |
Rabbit |
Isotype: |
IgG |
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