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ATG5 Polyclonal Antibody (CAB7252)

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SKU:
CAB7252
$331.89

Description

Overview

Title:ATG5 Polyclonal Antibody (CAB7252)
Size:100µL
Code:CAB7252
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene.

Immunogen Information

Immunogen:A synthetic peptide of human ATG5
GeneID:9474
SwissProt:Q9H1Y0
Synonyms:ATG5; APG5; APG5-LIKE; APG5L; ASP; hAPG5; autophagy protein 5
Calculated MW:22kDa/32kDa
Observed MW:54kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:10 - 1:100; IF 1:10 - 1:100
Storage Buffer:Store at 4°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:Mouse kidney
Cellular Location:Cytoplasm, Peripheral membrane protein, Preautophagosomal structure membrane

Antibody Images

Western blot - ATG5 Polyclonal Antibody (CAB7252)

Western blot analysis of extracts of mouse kidney, using ATG5 antibody (CAB7252).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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