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ATG4B Polyclonal Antibody (CAB2837)

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SKU:
CAB2837
€299.00

Description

Overview

Title:ATG4B Polyclonal Antibody (CAB2837)
Size:100µL
Code:CAB2837
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-393 of human ATG4B (NP_037457.3).
GeneID:23192
SwissProt:Q9Y4P1
Synonyms:ATG4B; APG4B; AUTL1; cysteine protease ATG4B
Calculated MW:31kDa/37kDa/42kDa/44kDa/52kDa
Observed MW:44kDa

Applications

Reactivity:Human
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:HepG2, BT-474, SKOV3, HeLa, Jurkat
Cellular Location:Cytoplasm

Antibody Images

Western blot - ATG4B Polyclonal Antibody (CAB2837)

Western blot analysis of extracts of various cell lines, using ATG4B antibody (CAB2837) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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