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ATG3 Polyclonal Antibody (CAB5809)

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CAB5809
  • Western blot - ATG3 Polyclonal Antibody (CAB5809)
  • Immunohistochemistry - ATG3 Polyclonal Antibody (CAB5809)
  • Immunohistochemistry - ATG3 Polyclonal Antibody (CAB5809)
$331.89

Description

Overview

Title:ATG3 Polyclonal Antibody (CAB5809)
Size:100µL
Code:CAB5809
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-314 of human ATG3 (NP_071933.2).
GeneID:64422
SwissProt:Q9NT62
Synonyms:ATG3; APG3; APG3-LIKE; APG3L; PC3-96; autophagy related 3
Calculated MW:35kDa
Observed MW:37kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:Raji, HeLa, Mouse liver, Mouse heart, Mouse skeletal muscle, Mouse kidney
Cellular Location:Cytoplasm

Antibody Images

Western blot - ATG3 Polyclonal Antibody (CAB5809)

Western blot analysis of extracts of various cell lines, using ATG3 antibody (CAB5809) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Immunohistochemistry - ATG3 Polyclonal Antibody (CAB5809)

Immunohistochemistry of paraffin-embedded mouse lung using ATG3 antibody (CAB5809) at dilution of 1:200 (40x lens).

Immunohistochemistry - ATG3 Polyclonal Antibody (CAB5809)

Immunohistochemistry of paraffin-embedded mouse spleen using ATG3 antibody (CAB5809) at dilution of 1:200 (40x lens).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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