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ATG16L1 Polyclonal Antibody (CAB1871)

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CAB1871
  • Western blot - ATG16L1 Polyclonal Antibody (CAB1871)
  • Immunofluorescence - ATG16L1 Polyclonal Antibody (CAB1871)
$331.89

Description

Overview

Title:ATG16L1 Polyclonal Antibody (CAB1871)
Size:100µL
Code:CAB1871
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 90-300 of human ATG16L1 (NP_110430.5).
GeneID:55054
SwissProt:Q676U5
Synonyms:ATG16L1; APG16L; ATG16A; ATG16L; IBD10; WDR30; autophagy related 16 like 1
Calculated MW:47kDa/49kDa/53kDa/66kDa/68kDa
Observed MW:68kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:U-937, 293T, HepG2, SH-SY5Y, SW620
Cellular Location:Cytoplasm, Peripheral membrane protein, Preautophagosomal structure membrane

Antibody Images

Western blot - ATG16L1 Polyclonal Antibody (CAB1871)

Western blot analysis of extracts of various cell lines, using ATG16L1 antibody (CAB1871) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence - ATG16L1 Polyclonal Antibody (CAB1871)

Immunofluorescence analysis of A-549 cells using ATG16L1 antibody (CAB1871). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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