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ATG14 Polyclonal Antibody (CAB7526)

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CAB7526
  • Western blot - ATG14 Polyclonal Antibody (CAB7526)
  • Immunohistochemistry - ATG14 Polyclonal Antibody (CAB7526)
$331.89

Description

Overview

Title:ATG14 Polyclonal Antibody (CAB7526)
Size:100µL
Code:CAB7526
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

N/A

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human ATG14 (NP_055739.2).
GeneID:22863
SwissProt:Q6ZNE5
Synonyms:ATG14; ATG14L; BARKOR; KIAA0831; autophagy related 14
Calculated MW:42kDa/55kDa
Observed MW:60kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:MCF-7, HeLa, Mouse liver, Mouse brain, Mouse ovary, Rat brain, Rat lung
Cellular Location:Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum membrane, Peripheral membrane protein, Preautophagosomal structure membrane, autophagosome membrane

Antibody Images

Western blot - ATG14 Polyclonal Antibody (CAB7526)

Western blot analysis of extracts of various cell lines, using ATG14 antibody (CAB7526) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Immunohistochemistry - ATG14 Polyclonal Antibody (CAB7526)

Immunohistochemistry of paraffin-embedded rat testis using ATG14 antibody (CAB7526) at dilution of 1:100 (40x lens).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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