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ATG13 Polyclonal Antibody (CAB0690)

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CAB0690
  • Western blot - ATG13 Polyclonal Antibody (CAB0690)
  • Immunohistochemistry - ATG13 Polyclonal Antibody (CAB0690)
  • Immunohistochemistry - ATG13 Polyclonal Antibody (CAB0690)
  • Immunofluorescence - ATG13 Polyclonal Antibody (CAB0690)
$331.89

Description

Overview

Title:ATG13 Polyclonal Antibody (CAB0690)
Size:100µL
Code:CAB0690
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is an autophagy factor and a target of the TOR kinase signaling pathway. The encoded protein is essential for autophagosome formation and mitophagy.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 281-480 of human ATG13 (NP_055556.2).
GeneID:9776
SwissProt:O75143
Synonyms:ATG13; KIAA0652; PARATARG8; autophagy related 13
Calculated MW:44kDa/52kDa/56kDa/60kDa
Observed MW:75kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:HeLa, A-549, NCI-H460, SH-SY5Y, Rat brain, Rat heart
Cellular Location:Cytoplasm, Preautophagosomal structure, cytosol

Antibody Images

Western blot - ATG13 Polyclonal Antibody (CAB0690)

Western blot analysis of extracts of various cell lines, using ATG13 antibody (CAB0690) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 10s.

Immunohistochemistry - ATG13 Polyclonal Antibody (CAB0690)

Immunohistochemistry of paraffin-embedded rat testis using ATG13 antibody (CAB0690) at dilution of 1:100 (40x lens).

Immunohistochemistry - ATG13 Polyclonal Antibody (CAB0690)

Immunohistochemistry of paraffin-embedded rat brain using ATG13 antibody (CAB0690) at dilution of 1:100 (40x lens).

Immunofluorescence - ATG13 Polyclonal Antibody (CAB0690)

Immunofluorescence analysis of A549 cells using ATG13 antibody (CAB0690). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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