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Asymmetric DiMethyl-Histone H3-R26 pAb (CAB2375)

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CAB2375
  • Western blot - Asymmetric DiMethyl-Histone H3-R26 pAb (CAB2375)
  • Immunofluorescence - Asymmetric DiMethyl-Histone H3-R26 pAb (CAB2375)
$476.19

Description

Overview

Title:Asymmetric DiMethyl-Histone H3-R26 pAb (CAB2375)
Size:100µL
Code:CAB2375
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.

Immunogen Information

Immunogen:A synthetic methylated peptide corresponding to residues surrounding Arg26 of human histone H3
GeneID:8356
SwissProt:P68431
Synonyms:HIST1H3J; H3/j; H3FJ; histone H3.1
Calculated MW:15kDa
Observed MW:18kDa

Applications

Reactivity:Human, Mouse, Rat, Other (Wide Range)
Tested Applications:WB; IHC; IF; IP
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200; IP 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive Samples:HeLa, H3 protein
Cellular Location:Chromosome, Nucleus

Antibody Images

Western blot - Asymmetric DiMethyl-Histone H3-R26 pAb (CAB2375)

Western blot analysis of extracts of various cell lines, using Asymmetric DiMethyl-Histone H3-R26 antibody (CAB2375).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence - Asymmetric DiMethyl-Histone H3-R26 pAb (CAB2375)

Immunofluorescence analysis of 293T cells using Asymmetric DiMethyl-Histone H3-R26 antibody (CAB2375). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Reactivity:
Other (Wide Range)
Host Species:
Rabbit
Isotype:
IgG
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